With one molecular PCR assay sample a result for targeted pathogens of Listeria mono., Salmonella spp., and E-Coli STEC/0157 can be determined in 24 hours or less. There is just one enrichment step per sample to get all 4 analytes amplified for PCR. Multiplex has been validated with AOAC PTM 041801 on matrices of deli meat turkey, raw ground beef, fresh baby spinach, and stainless steel environmentals. Internal validations were also done on matrices of raw beef trim, whole milk, brie de meaux, smoked salmon, peanut butter, raw eggs, black pepper, chicken meat, marinated poultry meat, turkey ham, pork meat, ground beef, sausages, raw milk cheese, infant formula, frozen broccoli, salad, pasteurized whole egg, whole egg powder, chocolate mousse, and milk.
Multiplex does not cover cronobacter sakazakii or campylobacter.